Omega test house has facilities for carrying out research and training program in the areas of biotechnology, microbiology, molecular biology chemistry, pharmaceutical sciences. Major equipment and facilities such as high performance liquid chromatography, (HPLC - Waters), Gas chromatography (G.C.), P.C.R., Lab scale Fermenter, Uv-vis-Spectrophotometer, D.N.A. & protein Electrophoresis system, high speed refrigerated centrifuge, Light microscope with projection system and (F.T.I.R.S & A AS - under acquisition)

Advanced Techniques Training

Six Weeks - (Fee Rs. 8000/- per Student)

INSTRUMENTATION UV/ visible spectrophotometer, PCR, Electrophoresis transilluminator, laminar air flow, microscope, pH meter, autoclave, centrifuge, shaker, water purification system, auto pipettes, weighing balance etc.

Basic knowledge of designing, performing and analyzing the experiments

Collection of samples from different sources using different techniques

Media preparation and sterilization techniques

Studying the type of microbial flora of different samples

Staining techniques (simple staining, differential staining, Gram staining, acid fast staining.

Screening of organisms for the production of enzyme producing isolates (Proteases, Cellulases, Xylanases, Amylases, Lipases, Mananases etc).

Shake flask fermentation for enzyme production.

Identification of isolates by biochemical methods.

Monitoring the growth and enzyme production.

Enzyme production at lab scale fermenter

Basic characterization of the enzyme.

Isolation of plasmid DNA

Isolation of genomic DNA

Agrose gel electrophorese.

Testing the pharma products eg Tablets, Capsules, Syrups, injections etc.

Introduction and principle of HPLC and its various components

Introduction to Gas Chromatography (GC).

Module A: Molecular Biology

Six Weeks - (Fee Rs. 8000/- per Student)

INSTRUMENTATION UV/visible spectrophotometer, laminar air flow, microscope, pH meter, autoclave, centrifuges, shakers, water purification system, auto pipettes, weighing balances, Electrophoresis apparatus, Transilluminater, PCR etc.

Introduction to recombinant DNA tools and genetic engineering techniques

General rules to perform molecular biology experiments

Preparation of solutions for the molecular biology experiments.

Isolation of plasmid, genomic, metagenomic DNA.

Agrose gel electrophoresis of isolated DNA.

Spectrophotometeric estimation of quantity and purity of isolated DNA.
Restriction digestion of DNA.
Ligation of fragmented DNA in the plasmid.
Trasformation and screening of recombinants
Selection of the recombinant of interest by masterand replica plating.
Designing of primers
PCR
Western Blotting
Plasmid curing
Analyses of the DNA sequences.
Retrieving different DNA sequences from the gene banks
Alignmnt of DNA sequences using different
softwares.
Analyses of the derived protein sequence using bioinformatics.

Module B: Microbiological Techniques

Four Weeks - (Fee Rs. 6000/- per Student)

INSTRUMENTATION UV/ visible spectrophotometer, laminar air flow, microscope, pH meter, auto claves, centrifuges, shakers, water purification system, auto pipettes, weighing balances etc.

Preparation of microbiological media (Basal medium, enrichment medium, selectionmedium etc.)

Sterilization techniques (Dry heat sterilization, moist heat sterilization, chemical sterilization).

Culture techniques (Spread plating, pour plating, streaking, stab cultures).

Shake flask fermentation for enzyme production.

Staining techniques (simple staining, differential staining, Gram staining, acid fast staining.

Wet mount and hanging drop preparation.

Identification of bacterial isolates by biochemical methods.

Preservation of microorganism .

Processing the different clinical samples.

Antibiotic sensitivity testing of different clinical samples.

Microbiological examination of water

Module C: Advanced Techniques

Three Weeks - (Fee Rs. 8000/- per Student)

(A)INTRODUCTION & PRINCIPLES of HPLC - G.C. AND THEIR COMPONENTS.

The various Modes of chromatographic separation(Reversed phase, Normal phase etc.)

Understanding working of HPLC, degassing the mobile phase and preparation of sample.

To control retention and selectivity in reversed-phase LC.Concept of gradient elution

Analytical method validation and troubleshooting

Introduction to gas chromatography(GC). Column types and selection. Sample introduction techniques

Reviewing , liquid, and other injection methods. FID detection systems

Analysis and interpretation of results. Routine maintenance and troubleshooting

(B)INTRODUCTION TO FERMENTER FUNCTIONING

Media formulation for enzyme production

Sterilization of fermenter, media and ancillary equipments

Demonstration of Fermenter functioning

Inoculation of media

Monitoring the growth &Enzyme production

Decontamination of fermenter & ancillary equipment

Module D: Pharmaceutical Chemistry / Chemistry

Four Weeks - (Fee Rs. 8000/- per Student)

INSTRUMENTATION HPLC, GC, UV-VIS spectrophotometer, dissolution app., pH meter, auto pipettes, weighing balance, centrifuge, water purification system etc.

Testing of pharma products eg. Tablets, capsules. Syrups, injections etc.

Wet chemical analysis of Pharma Products.

Qualitative/ Quantitative analysis of chemical sample

Practicals in Volumetric/ Gravimetric/ Colorimetric analysis Water analysis

Introduction & principle of HPLC and its various components. The various Modes of chromatographic separation(Reverse phase, Normal phase etc.)

Understanding working of HPLC, Degassing the mobile phase and Preparation of sample.

To control retention and selectivity in reversed-phase LC.Concept of gradient elution

Analytical method validation and troubleshooting

Introduction to gas chromatography (GC). Column types and selection. Sample introduction techniques

Reviewing of injection methods. FID detection system

Analysis and interpretation of results. Routine maintenance and troubleshooting

Module F: Food & Fermentation Technology

Four Weeks - (Fee Rs. 8000/- per Student)

INSTRUMENTATION UV/visible spectrophotometer,
laminer air flow, microscope, pH meter, autoclave,
centrifuge, shaker, water purification system, auto
pipettes, weighing balances etc.

To determine bacteriological quality of raw milk

MBR (Methylene Blue Reduction test)

Resazurin reduction test

SPC (Standard plate count)

DMC (direct microbial count)

Rapid plate form tests

To determine the bacteriological quality of various food products eg. Ice-cream, butter, condensed milk, etc.

Quantification of reducing, non reducing, and total sugar in various foods eg. Milk & milk products, fruit juice, etc.

Quantification of starch in various food products eg. Cereals.

Quantification of fat content in various food products.

Introductions to Fermentation and system types, Media formulation for enzyme production.

Sterilization of fermenter, media and ancillary equipments.

Demonstration of Fermenter functioning & process piping Inoculation of media.

Monitoring the growth & Enzyme production

Microbiological examination of water.

Module G: Project Work

Four Months (Fee Rs. 25000/- per Student for a group of minimum 5 students)

Molecular Biology

Microbiology

Advanced Techniques for the analysis of industrial products

Pharmaceutical Chemistry

Water Testing

Facilities exist for the longer term project work
in the above areas.

Summer Training And Project Work

We are offering for the students of BSC, MSC and B.TECH related to microbiology, biotechnology, chemistry, pharmaceutical microbial and food technology and allied sciences.

TRAINING & PROJECT WORK

We are offering for the students of BSC, MSC and B.TECH.

Omega Test House

Chemical Testing Services (1)

Civil Engineering Products Testing (1)

Microbiological Testing Services (1)

Non Destructive Testing (2)

Pharmaceutical Testing Services (1)

Summer Training And Project Work (9)

Testing For Civil Engineering Materials (1)

Projects

On Site Facility

Summer Training and Project Work