Bacterial Endotoxins Test also called as
Limulus amebocyte lysate (LAL) test is in vitro assay for detection and quantization of bacterial endotoxin. The test can be performed using the gel clot technique and the kinetic turbidimetric and chromogenic (colorimetric) assays. LAL Chromogenic endotoxin assay makes the use of modified Limulus Amoebocyte Lysate and a synthetic color-producing substrate to detect endotoxin presence.
This assay is quantitative and the color intensity developed upon addition of the sample to the LAL supplied with the kit is proportional to the amount of endotoxin present in the sample and can be calculated from a standard curve. The kinetic chromogenic quantitative assay for bacterial endotoxin can be performed at the low assay range (0-5 EU/ml) or the higher range (5-50 EU/ml) depending on sponsor's request.
The assay is performed using a Molecular Devices Microplate reader controlled by a software. During the 60 minute incubation, readings are collected from all wells. The standard curve is deduced from the 241 readings and sample concentrations are calculated automatically based on the standard curve. The assay is highly sensitive.
- Lysate- 0.125 / 0.06 /0.03 sensitivity
- Control Standard Endotoxin,
- LAL REAGENT WATER,Micro tips,
- 10 X 75mm Reaction Tubes,
- 16 X 100mm Dilution tubes Non- Depyrogenated,
- Heating Block,
- Vortex mixture,
- 10 X 75mm PYROGEN FREE Reaction Tubes
- 16 X 100mm PYROGEN FREE Dilution tubes
