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Double Helix is a knowledge based laboratory where its scientific team is constantly abreast with the new advancements in reproductive immunology and is prompt to procreate the study which thus converts the scientific discovery into meaningful application. It offers cutting edge services in the realms of Clinical Cytogenetics, Reproductive Immunology and Stem cells. With the growing awareness about the treatment regimes through Stem Cells, very soon the field of Medicine would be taken under the canopy of Stem Cell therapy which would thus offer applicable translational treatment regimes for various autoimmune disorders. Autoimmunity is basically the rejection of the ‘self’ which corresponds to reproductive failure. Our treatment regimes have benefitted patients with autoimmune disorders (Rheumatoid arthritis, Parkinson’s disease, etc.). As the foetus is an allograft in the mother’s body; its rejection is the result of the triggering of autoimmunity thus classifying reproductive failure as an autoimmune disorder.+ Read More

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Molecular Testing
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Molecular Testing

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  • Y – Chromosome microdeletion
  • DNA Paternity testing

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Diagnostic Testing
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Diagnostic Testing

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Infertility and Recurrent Miscarriages
The importance of Immune Pathological Evaluation of Pregnancies that Terminated in Spontaneous Miscarriage.
 
When a pregnancy fails, women often have a Dilatation and Curettage (D and C). When pregnancy tissue is obtained, it is sent to a pathologist usually associated with a laboratory or a hospital. Once the tissue is received, it is fixed in a formalin solution and embedded in paraffin (the final product is a paraffin block). The pathologist cuts the block into small slices, stains the tissue in the paraffin and looks at it under the microscope to determine that the tissue is pregnancy tissue and that it is normal and not infected. This is usually all that is done. Our Laboratory can do further testing (placental immunopathology) to determine if there is immune problem.

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Immune Testing
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Immune Testing

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One is advised to opt for the immune testing when, she has:

(a) undergone two miscarriages or two IVF failures after the age of 35 years OR three
      miscarriages or three IVF failures before the age of 35 years,
(b) poor egg production (less than 6 eggs) from a stimulated cycle,
(c) blighted ovum,
(d) idiopathic (unknown cause) infertility,
(e) previous autoimmune problems (ANA positive, rheumatoid arthritis, and/or lupus),
(f) experienced previous pregnancies that have shown retarded fetal growth, and
(g) one living child and repeated miscarriages while seeking to have a second child.

For addressing the issue of recurrent miscarriage, it becomes a pre-requisite to identify the cause of the patient’s recurrent reproductive failures through an array of various reproductive immunological tests. In order to put the patient on the fertility treatment regime, his/ her prior extensive testing is performed followed by its corresponding diagnosis. The reproductive failure may be the result of either immunologic causes or non-immunologic causes. The assessment of immunologic causes can be described as a function of any of the following three conditions:
  • Presence of any autoimmune disease, endometriosis or Polycystic Ovarian Syndrome (PCOS) in the mother.
  • Rejection of the immune system due to the failure of the embryo to generate immune tolerance with non-existence of any inflammatory precondition.
  • The coupling of the above two conditions.
We offer treatment regime not solely to help our patients conceive but we also perform proper monitoring of the patient’s pregnancy. The tests may be conducted at various phases as described below:

a. For determination of the appropriate treatment regime
b. After the treatment but before planning for pregnancy
c. In case of miscarriage or IVF failure
d. For assessing the progress of the conceptus as well as the pregnancy

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Cytogenetic Testing
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Cytogenetic Testing

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  • Peripheral Blood (Couple/ Individual)
  • Cord Blood
  • Haematologic disorders (Chromosomal analysis in CML, AML, APML, ALL)
  • Chromosome breakage studies-Fanconi Anemia
  • Products of Conception
  • Fragile X Chromosome analysis
  • Chorionic Villus Biopsy
  • Amniotic Fluid.

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Stem Cell Therapy Services
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The domain of Regenerative medicine pertains to the innovative clinical therapies aiding in the acceleration of the healing of the lost or damaged tissues/ organs. This field promises to be a boon for human tissue or organ transplantation. The last two decades have witnessed a tremendous advancement in the field of regenerative medicine which has transformed from a toddler to a young adult. The ambiguity surrounding the field has gradually faded away. Today the Regenerative medicine has reached a stage where it has been put to application based on the convincing results yielded by the ongoing research. Till date whatever has been learnt about regenerative medicine is just the tip of the iceberg.

The regenerative medicine therapies are devised to extract benefit from the body’s healing abilities. In cases of autoimmune disorders (Type I Diabetes, rheumatoid arthritis, Parkinson’s disease, etc.) where the body fails to recognize its own cells as ‘self’, regenerative medicine has proved to be a blessing. With a promise to cure and not just treat a disease, this new frontier of medicine has entrusted people’s faith in medicine for diseases that have been classified as incurable. Thus regenerative medicine indeed flags the paradigm shift in the sphere of healthcare.

We ourselves have witnessed therapeutic success from the regenerative medicine. The mesenchymal stem cells and its products tend to reverse the levels of the immunological factors causing autoimmune disorders, thus benefitting the patients to a large extent.
Here at Double Helix, the stem cells have been used to effectively treat patients suffering from:

  • Vitiligo
  • Parkinson’s Disease
  • Multiple Sclerosis
  • Rheumatoid Arthritis
  • Cerebral Palsy
  • Type 2 Diabetes Mellitus
  • Chronic Kidney Disease
  • Spino-cerebellar ataxia
  • Progressive Supranuclear Palsy
  • Metastasis in Lungs and Liver
  • Hepatocellular carcinoma liver
  • Neural injury with cardiac arrest and retinitis pigmentosa
  • Squamous cell carcinoma of lung
  • Attention Deficit Hyperactivity Disorder (ADHD)
  • Low IQ/ Mental retardation
  • Hypoimmunoglobinaemia
  • Interstitial lung disease
  • Down’s Syndrome
  • End stage Renal Disease
  • High functioning Asperger’s syndrome
  • Chronic arthritis and chronic gastritis
  • Multiple system atrophy
  • Chronic sleep syndrome
  • Autism
  • Becker’s Muscular Dystrophy
  • Creutzfeldt Jacob Disease

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Reproductive Immunology Testing
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  • N.K. cells (Blood/ Tissue)
  • Progesterone Receptor (Tissue)
  • PDF (Blood/ Tissue)
  • TNF (Blood/ Tissue)
  • Macrophage (Tissue)
  • Latent Tuberculosis Detection (Blood).

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