Traditionally, there were several methods to measure viable cells viz Trypan blue staining, tritium-labeled thymidine (radioactive substances was used and it was time consuming).
Thus, MTT assay is most commonly used for measuring viability testing.
Yellow MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide, a tetrazole) is reduced to purple formazan in the mitochondria of living cells. This reduction takes place only when mitochondrial reductase enzymes are active, and therefore conversion can be directly related to the number of viable (living) cells.
Shree Krushna Gene Lab and Research Centre supports genetic diagnosis and research on the human and model genomes and on all aspects of genetics, basic biochemistry and cell biology related to health and disease, including the translation of knowledge into health policy and practice, and the societal implications of genetic discoveries.
Shree Krushna Gene Lab and Research Centre design for Cytogenetic and molecular genetic diagnosis cum research full pledge laboratory. Each test is tested by trained and experienced technicians under the supervision of qualified Scientist.
Predictive Toxicology Testing:
Toxicology profile with range in primary profiling and complete profiling of any novel, synthetic and analogue compound of commercial, pharmaceutical, industrial or any part of health sector are served according to need. (OECD guideline ) The wide range allows selective or complete profile study of compound depending upon the purpose of use and application. The few services under this portfolio are:1. In Vitro Toxicity assay 2. In Vitro Geno Toxicity assay 3. Molecular Genotoxicity assay 4. Environmental toxicity 5. Drug/ Chemical / Dye genotoxicity 6. Cancer genetics
OECD 473 : “In Vitro Mammalian Chromosome Aberration Test”
OECD 479 : “Genetic Toxicology: In vitro Sister Chromatid Exchange
Assay in Mammalian Cells”
OECD 487 : “In Vitro Micronucleus Test”
OECD/Guideline : “COMET assay – Single cell electrophoresis”
OECD/Guideline : “MTT assay” Cell Viability testing.
OECD/Guideline : “Trypan blue” Cell Viability testing.
OECD 471 : “AMES Test – Bacterial Reverse Mutation Test”