Sanzyme Biologics Private Limited

Uni Ecosense is a synergistic blend of 6 probiotic strains which is a flagship product of Sanzyme Biologics used in Aquaculture. Uni Ecosense contains a proprietary blend of 6 Bacillus species. It has been studied for its benefits and performance in aquaculture in both the lab and field trials. More than 50 tonnes of the product is applied in 6 countries with consistent results.

Water quality and disease free environment is the key factor for successful shrimp culture. Organic waste accumulation due to the left over feed, fecal matter and dead planktons contributes to the proliferation of pathogenic microbes like Vibrio sp. Vibrio sp are the common bacterial species which are found in large quantities that can cause septicemia and affect the digestive system of the shrimps which is collectively called as Vibriosis. The high loads of Vibrio leads to severe infections which will evoke mass mortalities in the farmed communities leading to heavy monetary losses.

Key Benefits:

•         Uni Ecosense produces a variety of anti bacterial substances which effectively controls Vibrio loads in water.
•         Uni Ecosense produces enzymes like amylase, protease, lipase and cellulase which digests the organic waste that is food for harmful phytoplanktons thus encouraging the beneficial phytoplanktons of the pond water and also maintains the water pH and alkalinity.

Lab Trial:

Objective:

        Reduction of Vibrio loads in water.

Design:

•         2 tanks of 5 Lt saline water is taken for trial.
•         20 ml of Vibrio culture (124 X 106 CFU / ml) is added to each tank.
•         24 hours after the application of Vibrio culture the sample is applied to the test tank.
•         300 gms per acre dose is calculated and  applied in test tank except control.
•         Before application of the product, the water samples are drawn for initial Vibrio count.
•         After 48 hours of the product application the water samples are drawn for Vibrio count. The same is repeated for 72 hours and 96 hours.
•         The 96 hours samples are the final results of the product.

Results:

The results clearly indicate the reduction of Vibrio loads in the test tank when compared to the control.

Field Trial:

Objective:

•         Reduction of Vibrio loads in water.
•         Density of phytoplankton before and after application of the product.

Design:

2 earthen ponds with 0.5 a / pond with stocking density of 75 pcs / M2 were taken for trial, in which one pond was applied with Uni Ecosense and another was used as a control. The dosage of 300 g / acre was calculated and applied to the test pond.

Trial Parameters:

•         Vibrio sp. Analysis before and after application of the product. The maximum limit is Green colonies below 100 CFU/ml.
•         Density of phytoplankton before and after application of the product. Density of Phytoplanktons is measured by Secchi disk method.
  

Sanero Ammonox is a synergistic blend of carefully selected probiotic sp which are capable of nitrification and denitrification activities. Sanero Ammonox is a flag ship product of Sanzyme Biologics which is used in Aquaculture. It has beed studied for its benefits and performance in aquaculture in lab and field trials. More than 15 tonnes of the product is applied in 6 countries with consistent performance.

Shrimp feed is rich in protein content, when the left over feed, digested feed in the form of fecal matter and dead planktons accumulates in the pond environment the proteins degrades to form the nitrogenous compounds. The nitrogenous compounds are in the form of ammonia and nitrites which interfere in the oxygen displacement in water and affect the shrimp haemolymph in interfering the oxygen displacement leading to respiratory diseases and creates mass mortality in the shrimps causing heavy monetary losses to the farmers.

Key Benefits:

•     Sanero Ammonox speedups the denitrification cycle and converts the noxoius gases to harmless nitrogen products.
•     Sanero Ammonox precipitates the ammonia and gives an instant relief from the stress.
•     Sanero Ammonox absorbs H2S, ammonia and nitrites.
•     Sanero Ammonox probiotics improves the water quality and maintains the pH of the pond.
•     Sanero Ammonox reduces the ammonia toxicity in the gut when administered in feed.
•     Sanero Ammonox acts as a feed supplement in enhancing the growth of the cultured species and improves the growth of beneficial planktons.

Lab Trial:
Objective:

•     Reduction of Ammonia in pond water.
•     Reduction of nitrites in pond water.

Design:

•     2 tanks of 5 Lt saline water is taken for trial.
•     Ammonia and nitrites were artificially induced in to the system at a level of 1.2 ppm of total ammonia and 0.5 ppm of nitrite.
•     A dose of 1 lit / acre was calculated and applied in the test tank.
•     No addition of the product was done in control tank.
•     After 48 hours of application of the product the samples were drawn for the ammonia and nitrite reduction.
•     The 72 hours samples were considered as final results of the product.

Results:

The results have indicated the reduction of ammonia and nitrites in the test tank when compared with the control tanks.

Field Trial:
Objective:

•     Reduction of Ammonia in pond water
•     Reduction of nitrites in pond water.

Design:

Two earthen shrimp ponds (1.5 acre/pond) with a depth of 1-1.2 m were stocked with juvenile shrimp  at a density of 75 shrimp/m² in which our product Sanero Ammonox applies in one pond  and  one pond kept as a control. A dose of 1 L per acre was calculated and applied to the test pond.

Trial Parameters:

•     Monitoring of ammonia level before and after application of the product. Tolerable limit of ammonia is Total Ammonia <1.0 ppm & Free Ammonia <0.1 ppm.
•     Monitoring of Nitrites level before and after application of the product. Tolerable limit of Nitrite is below 0.25 ppm