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Biotechnology is the scientific art of utilizing biological entities to make desirable products for enhancing human welfare. This novel approach hence serves as an exciting, revolutionary, scientific discipline and has emerged as a dominant economic sector globally. Biotechnology deals with integral applications of gene technology, tissue culture, enzymology, immunotechnology, metabolic engineering, microbiology, agriculture, pharmaceutical technology, commercialization of molecular biotechnology and process engineering techniques in manufacturing and service industries. The Director Shri Shreedhar G. Bhat has acquired long experience in Molecular biology and Plant Tissue Culture. Within a limited period “Dedicated team’ of SBL has trained many students of various steams like B.Sc., M.Sc., B.E, B. Tech, M.Tech, B. Pharm from different colleges and universities in various aspects of Biotechnology.
The laboratory facilities have been used by the M.Phil candidates to carry out their projects/dissertation work at SBL. SBL has been recognized by “Karnataka Biotechnology and Information Technology Services (KBITS)” as a Biotech Industry and has created a benchmark by synthesizing its own products
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Genetic Engineering

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In addition to all the contents of Project-I, this will have the following aspects:

  • Screening of transformants by restriction analysis
  • Transferring the gene constructs in binary vectors to Agrobacterium through triparental mating (TPM)
  • Transferring of gene to plant system through Agrobacterium mediated plant transformation
  • Leaf disc preparation
  • Co-cultivation
  • Selection media transfer
  • Analysis of transgenic plants through PCR for confirming the presence of gene
  • Plant Genomic DNA extraction
  • Polymerase Chain Reaction

 

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Plant Tissue Culture

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  • Plant tissue culture media preparation and autoclaving
  • Cleaning and sterilization procedures
  • Isolation of embryos from the seeds and initiation
  • Sub-culturing & Node-culture
  • Synthetic Seed Preparation
  • Agrobacterium mediated plant transformation
  • Leaf disc preparation
  • Co-cultivation
  • Selection media transfer

 

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Last modified by IM/User on: 25 Mar 2014
  • Basic Handling
  • Handling of equipments properly
  • Basic calculations in molecular biology
  • Media preparation and autoclaving
  • Growth and maintenance of bacteria carrying recombinant plasmid
  • Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content
  • Restriction analysis
  • Gel elution of both vector and insert DNAs Separately
  • Ligation reaction
  • Competent cells preparation and transformation
  • Competent cells efficiency testing using known quantity of DNA
  • Screening of transformants through a-complementation test

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  1. Basic Handling: 
    •   Handling of equipments.
    • Basic calculations of molecular biology.
    • Media preparation and autoclaving.
  2. Growth and mantenance of bacteria carying recombinance of bactria.
  3. Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content.
  4. Restriction Digestion of RNA content.
  5. Gel elution of both vector and insert DNA saparately.
  6. Ligation reaction.
  7. Competent cell efficiency testion using known quantity of DNA

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Genetic Engineering

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In addition to all the contents of Project-I, this will have the following aspects :
  • Screening of transformants by restriction analysis.
  • Transferring the gene constructs in binary vectors to Agrobacterium through triparental mating (TPM)
  • Transferring of gene to plant system through Agrobacterium mediated plant transformation
    • Leaf disc preparation
    • Co-cultivation
    • Selection media transfer
  • Analysis of transgenic plants through PCR for confirming the presence of gene
    • Plant Genomic DNA extraction
    • Polymerase Chain Reaction

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Yes, I am interested!

Plant Tissue Culture

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  • Plant tissue culture media preparation and autoclaving
  • Cleaning and sterilization procedures
  • Isolation of embryos from the seeds and initiation
  • Sub-culturing & Node-culture
  • Synthetic Seed Preparation
  • Agrobacterium mediated plant transformation
    • Leaf disc preparation
    • Co-cultivation
    • Selection media transfer

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Immunological Studies

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  1. Extraction of total Proteins from different sources.
  2. SDS-PAGE for running protein samples from different sources including puified virus.
  3. Western blotting for immuno detection test.
  4. ELISA for testing the samples for viral (ToMV) infectoion.

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  • Regeneration system standardization of different medicinal plants (5 different topics are available)
  • Purification and characterization of mosaic virus (ToMV)
  • Commercial plant tissue culture intiation & multiplication of different crops
  • Identification of male female & hybrid in a given seed lot (hybrid seed purity test)
  • Whether it is research project or training project, We will guide you but you have to handle everything to get ‘hands on’ experience
  • During the training period, you may have to demonstrate a few experiment to your junior friends
  • There is no fixed duration for this training programme but you have to practice all the experiments and produce the results before completion
  • For practicals, each batch will have a maximum of six students
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