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Our product range includes a wide range of Genetic Engineering, Plant Tissue Culture, Recombinant DNA Technology, Recombinant Dna Technology, Immunological Studies and Project M.phil., M.sc., B.e., B.tech & Other Students.

Genetic Engineering

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In addition to all the contents of Project-I, this will have the following aspects:

  • Screening of transformants by restriction analysis
  • Transferring the gene constructs in binary vectors to Agrobacterium through triparental mating (TPM)
  • Transferring of gene to plant system through Agrobacterium mediated plant transformation
  • Leaf disc preparation
  • Co-cultivation
  • Selection media transfer
  • Analysis of transgenic plants through PCR for confirming the presence of gene
  • Plant Genomic DNA extraction
  • Polymerase Chain Reaction

 

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Plant Tissue Culture

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  • Plant tissue culture media preparation and autoclaving
  • Cleaning and sterilization procedures
  • Isolation of embryos from the seeds and initiation
  • Sub-culturing & Node-culture
  • Synthetic Seed Preparation
  • Agrobacterium mediated plant transformation
  • Leaf disc preparation
  • Co-cultivation
  • Selection media transfer

 

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Last modified by IM/User on: 25 Mar 2014
  • Basic Handling
  • Handling of equipments properly
  • Basic calculations in molecular biology
  • Media preparation and autoclaving
  • Growth and maintenance of bacteria carrying recombinant plasmid
  • Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content
  • Restriction analysis
  • Gel elution of both vector and insert DNAs Separately
  • Ligation reaction
  • Competent cells preparation and transformation
  • Competent cells efficiency testing using known quantity of DNA
  • Screening of transformants through a-complementation test

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  1. Basic Handling: 
    •   Handling of equipments.
    • Basic calculations of molecular biology.
    • Media preparation and autoclaving.
  2. Growth and mantenance of bacteria carying recombinance of bactria.
  3. Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content.
  4. Restriction Digestion of RNA content.
  5. Gel elution of both vector and insert DNA saparately.
  6. Ligation reaction.
  7. Competent cell efficiency testion using known quantity of DNA

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Genetic Engineering

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In addition to all the contents of Project-I, this will have the following aspects :
  • Screening of transformants by restriction analysis.
  • Transferring the gene constructs in binary vectors to Agrobacterium through triparental mating (TPM)
  • Transferring of gene to plant system through Agrobacterium mediated plant transformation
    • Leaf disc preparation
    • Co-cultivation
    • Selection media transfer
  • Analysis of transgenic plants through PCR for confirming the presence of gene
    • Plant Genomic DNA extraction
    • Polymerase Chain Reaction

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Yes, I am interested!

Plant Tissue Culture

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  • Plant tissue culture media preparation and autoclaving
  • Cleaning and sterilization procedures
  • Isolation of embryos from the seeds and initiation
  • Sub-culturing & Node-culture
  • Synthetic Seed Preparation
  • Agrobacterium mediated plant transformation
    • Leaf disc preparation
    • Co-cultivation
    • Selection media transfer

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Yes, I am interested!

Immunological Studies

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  1. Extraction of total Proteins from different sources.
  2. SDS-PAGE for running protein samples from different sources including puified virus.
  3. Western blotting for immuno detection test.
  4. ELISA for testing the samples for viral (ToMV) infectoion.

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  • Regeneration system standardization of different medicinal plants (5 different topics are available)
  • Purification and characterization of mosaic virus (ToMV)
  • Commercial plant tissue culture intiation & multiplication of different crops
  • Identification of male female & hybrid in a given seed lot (hybrid seed purity test)
  • Whether it is research project or training project, We will guide you but you have to handle everything to get ‘hands on’ experience
  • During the training period, you may have to demonstrate a few experiment to your junior friends
  • There is no fixed duration for this training programme but you have to practice all the experiments and produce the results before completion
  • For practicals, each batch will have a maximum of six students
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    Immunological Studies

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    • Extraction of total Proteins from different sources
    • SDS - PAGE for running protein samples from different sources including purified virus
    • Western blotting for immune detection test
    • ELISA for testing the samples for viral (To MV) infection

     

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    Yes, I am interested!

    • Projects for M.Phil., M.Sc., B.E., B.Tech & Other Students are being carried out in the fields of
    • Production and screening of transgenic plants with particular gene of interest (8 different topics are available)
    • Regeneration system standardization of different medicinal plants (5 different topics are available)
    • Varietal identification through DNA finger printing - (a) Chilli - (b) Tobacco
    • Purification and characterization of mosaic virus (ToMV)
    • Purification of Enzyme - (a) Taq DNA Polymerase - (b) Amylase
    • Identification of male female & hybrid in a given seed lot (hybrid seed purity test)
    • Biodegradation of organic material by micro organisms
    • Commercial plant tissue culture intiation & multiplication of different crops
    • Duration of 1.5 to 6 months
    • Fee Amount : Rs.10,000 to Rs.20,000
    • Whether it is research project or training project, We will guide you but you have to handle everything to get ‘hands on’ experience
    • During the training period, you may have to demonstrate a few experiment to your junior friendsThere is no fixed duration for this training programme but you have to practice all the experiments and produce the results before 
    • completion
    • For practicals, each batch will have a maximum of six students

     

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    Yes, I am interested!

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